Genetic_Mice

Genetic Mice Transgenic Outline Introduction Experimental Timeline Plan the Experiment Clone the Transgene Transgene Screening Transgene Expression DNA Microinjection Identify Transgenic Founders Southern Analysis Transgenic Analysis Pertinent Publications Review Articles and Books 1. Introduction. This is a brief outline of the steps necessary to obtain a transgenic mice. Simply put, the investigator constructs a transgene with a promoter and a structural gene (for example a reporter gene such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice are born. Transgenic founders are identified and bred to produce offspring for analysis. Core personnel are available for consultation on all aspects of transgenic research. Contact Thom Saunders with any questions or Meet the Staff. 2. Plan the experiment. What is the purpose of your experiment' Do you want to analyze tissue specific regulatory sequences' Do you want to overexpress a protein in a specific cell lineage' You will need to obtain or clone the desired promoter and structural gene. Certain genes will be deleterious or incompatible with proper growth and development of the embryo, special arrangements should be made if you expect embryonic lethality from transgene expression. The transgenic, transgene, dna, expression, mice, gene, assay, microinjection, analysis, should, core, weeks, vector, promoter, pcr, mouse, tail, founders, eggs, digest, determine, blot, before, verify, specific, southern, reporter, pups, protein, order, investigator, experiment, establish, construct, cloning, breeding, available, unique, tissue, time, tested, structural, sites, site, simply, service, sequences, screening, restriction