DNA_Hybridization

DNA Hybridization In 1960, Julius Marmur and his coworkers conducted studies at Harvard University that resulted in the discovery of the process of reannealing. This process involves the reassociation of single strands into double-stranded molecules that are stable. From the discovery of the reannealing process, the methodology nucleic acid hybridization has evolved. Using this technique, strands of nucleic acids that are both complementary and from different sources can be mixed to form double-stranded molecules which are labeled "hybrid" (Karp 429). Since its development, nucleic acid hybridization has branched into several different techniques and is used for a variety of purposes. There are a variety of purposes that can be fulfilled by nucleic acid hybridization. For example, a particular DNA probe (pDT1720) can be used to detect the presence of Campylobacter jejuni in food samples (Kingombe 4558). This probe, a particular nucleic acid, functions to identify DNA strands which are complementary to itself in sequence (Karcher 269). Also, "in situ hybridization" has been developed to detect the location of satellite DNA. In this method, the DNA of the chromosomes is held in place to allow it to react with a certain DNA dna, species, hybridization, each, strands, two, different, technique, hybrid, between, process, nucleic, molecules, double-stranded, because, type, tracer, temperature, sibley, mixtures, involves, human, form, driver, degrees, complementary, acid, study, single, similar, sequence, related, purposes, percent, karp, hybrids, humans, helices, comparison, closely, birds, ancient, water, vial, variety, using, useful, treatments, time