Microbiology_Lab

Microbiology Lab Natalie Robinson Dr. Farrah 0997-0870 Microbiology Lab Experiments Table of Contents Titration of Bacterial Virus Suspension (8/27)………………………………….1 Concentration of Viruses (9/1)…………………………………………………..3 Propagation of Animal Cells (9/8)………………………………………………4 Detection of Polovirus (9/15)……………………………………………………5 High Resolution Pictures…………………………………………………….......6 Apendixes……………………………………………………………………...…7 Tables…………………………………………………………………………….9 Titration of Bacterial Virus Suspension August 27 Purpose A method to determine a rough virus concentration in a suspension is usually determined by a method called plaque assay. This method is when a bacterial species is plated to completely cover the media on a Petri dish. Within this bacteria mixture a percentage of the original virus suspension is added. This percentage is called a dilution factor which is a ten fold dilution to produce a range from concentrated to dilute. The general concept of this procedure is the virus will infect some of the bacteria. The infected bacteria will be lysed leaving a hole in a confluent bacterial layer. Each hole will represent one virus. So after counting the plaques, holes, the original concentration can be calculated based on plaques counted, volume pipetted and the dilution factor. Results The plaques counted for each group are in graph 1. My personal results can be found in group D, number 13. I had a concentration of approximately 5.1e4. My group had a mean of 56 and a standard deviation of viruses, cells, group, concentration, standard, results, each, deviation, original, virus, contact, calculated, within, mean, inhibition, animal, purpose, plaques, monolayer, individual, grow, flocs, experiment, discussion, determined, close, added, show, september, range, presence, plaque, percentage, number