Optimal_Temp_And_PH_Of_Enzymes

Optimal Temp And PH Of Enzymes Determining the optimal temperature and pH of alpha-amylase and starch solution Abstract A catalyst is the chemical agent that changes the rate of a reaction without being consumed by the reaction. A cell’s physical and chemical environment affects enzyme activity. The activity of an enzyme is affected by general environmental factors, such as temperature and pH. Because the three-dimensional structures of proteins are sensitive, an enzyme has conditions under which it works optimally. Using absorbance readings, reaction rate and optimal levels can be concluded. The purpose of this experiment is to observe for 20 minutes the effect of temperature and pH on the reaction of α-amylase with starch and to establish the optimum temperature and pH for this reaction. I hypothesize that the optimal temperature and pH will be 30° C and 4.5, respectively. After setting the spectrometer to 560 nm, the temperature blank solution was prepared. It consisted of 0.1 ml of I2KI and 5 ml of distilled water in a glass cuvette. The spectrometer was calibrated with the blank cuvette. Then I prepared a mixture of 35 ml of starch and 35 ml of distilled water in a flask. I placed this ml, reaction, temperature, ph, solution, enzyme, optimal, absorbance, spectrometer, rate, experiment, one, i2ki, cuvettes, 70, 11, water, minute, after, 20, measured, #945-amylase, temperatures, starch, prepared, placed, minutes, five, cuvette, blank, barley, 45°c, 35, time, plotted