Cells

Cells Since myosin has a molecular weight of about 200,000 Dalton, it would be expected to be at the top of the gel (not far from the well, on the other hand, actin which have a molecular weight of about 42,000 Daltons, would be further from the top. Hence, it is much smaller and will move away on the graph when plotted. Based on my results, as the concentration of ATP increases, so does the contraction increases as well. There were also noticeable changes in the length of muscle when ADP was added. The same result was observed as with ATP; as the concentration of ADP increases so does the contraction. Muscle cells have enzyme which not only breaks down ATP to ADP (creatin), but can also make ATP from ADP by using phosphokinase. Myokinase also produce ATP. ADP + ADP ------------ ATP + AMP. ATP is regenerated by process such as glycolysis and mitochondrial oxidative phosphorylation. In muscle cell, the enzyme creatin kinase and creatin phosphate aid in this process. The difference between a contracted muscle and a relaxed muscle is; a contracted muscle has the maximum overlap between the actin and myosin filaments. However, there is no overlap at the antibody, primary, muscle, cells, cell, atp, actin, protein, enzyme, control, antibodies, adp, strip, stained, secondary, fluorescent, rabbit, non-specific, method, made, filaments, experimental, binding, visible, top, thin, specific, site, since, seen, nitrocellulose, much, motility, monoclonal, molecule