C-Fern_Lab

C-Fern Lab Inheritance of genes that code for the phenotypic color in Ceratopteris Abstract In this lab we took a green F1 diploid sporophyte population and allowed meiosis to occur to produce G2 haplolid gametophytes. Of the two cultures, Hybrid A gametophytes were produced in equal numbers of both mutants, while Hybrid B produced significantly more pale. These populations were allowed to fertilize randomly to produce a diploid F2 sporophyte population. Because of this diploidization, I hypothesized that there would be many more green sporophytes than pale ones in Hybrid A, since green is dominant (Lab Manual 2.2). I made this assumption believing that Hybrid A would follow the expected Mendelian ratio of a monohybrid cross, which is 3:1 (Klug & Cummings 46). The results obtained for Hybrid A held true to this hypothesis. Hybrid B followed a different pattern of inheritance (Lab Manual 2.2). After I obtained the class results for the F1 and F2 populations I hypothesized that the pattern of inheritance for Hybrid B was due to epistasis of one of the alleles in a dihybrid cross. The results of Hybrid B held true to the Mendelian ratio for dihybrid crosses, hybrid, green, results, class, pale, one, observed, gametophytes, expected, cross, dihybrid, table, alleles, sporophytes, plates, lab, two, hypothesis, dominant, week, sporophyte, ratio, fertilization, f2, diploid, data, chi-square, c-fern, allele, since, recessive, population, monohybrid, mendelian, haploid