Bioluciferase_Lab_Report

Bioluciferase Lab Report Bioluciferase, with no presence of mitochondria, is highly reactive among ATP, but altering pH levels results in denaturing Abstract By measuring how different reagents affect the luciferin and luciferase mixture, called lantern, we will show the importance of the energy contained in ATP as well as other reagents like ADP and GTP. Reactions, here, will occur due to enzyme specificity. If the active site of the lantern can be fit with the substrates we add, energy will be released in the form of light. We will then see how altering pH levels will affect the lantern by adding an acid as well as a base. NADH will be added to another tube to see how it will affect the lantern. NADH builds in mitochondria, and mitochondria helps to make ATP. So, by adding NADH, if there is a reaction we know that there are mitochondria present in the lantern. We filled seven vials with lantern and then added the reagents. Once all groups added all components to the vials we turned off the lights to see the light energy released. We rated our observances on a scale from zero to five, zero biouciferase, atp, lantern, energy, light, reaction, active, one, five, mitochondria, groups, release, therefore, site, results, ph, rated, nadh, gtp, group, experiment, reagents, luciferase, added, test, reactions, level, however, fit, because, base, zero, tube, shape, seven, produced