Bio_Lab_Report_On_The_Enviromental_Effects_Of_Enyzyme_Catzly

Bio Lab Report On The Enviromental Effects Of Enyzyme Catzlyzed Reaction Abstract: The effects of enzyme and substrate concentration, temperature and pH on catecholase activity were determined in this experiment. To attain these results, potato extract (Solanum tuberosum) was mixed with catechol and exposed to different environmental conditions, then placed in a spectrophotometer to measure and record the different absorbance readings at a 420nm wavelength. It was found that catecholase activity was optimal with a pH of 6.6 and in temperatures between 27-30 ºC. The enzyme and the substrate concentrations affected the rate of reaction by increasing it. Introduction: Enzymes are a kind of protein that catalyze reactions by bonding to specifically shaped active sites thus lowering the energy reaction level which in turn speeds up the reaction. Catecholase or polyphenoloxidase (PPO), an enzyme found in various fruits and vegetables, converts catechol to benzoquinone and water in the presence of oxygen. (Mitchell et al., 2002) Fruits and vegetables use this process to protect themselves from rotting and bacteria. (Wegener, 2002) In this experiment we focused on the effect enzyme concentration, substrate concentration, temperature, and pH, had on the enzyme activity of catecholase (in this experiment potato juice was used). We hypothesized that changing the temperature and pH from within the optimal reaction, enzyme, ph, rate, experiment, substrate, temperature, 2002, tubes, 1ml, ºc, concentration, catechol, water, minutes, potato, absorbance, test, taken, spectrophotometer, readings, prepared, optimal, juice, experimental, catecholase, amount, added, , same, results, phosphate, every, et, effect, changes